human melanoma cell line m10 Search Results


97
ATCC m10 m1 müller glial cells
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ATCC source sex
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Novus Biologicals osr1
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
Osr1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioResource International Inc human breast epithelial cell h184b5f5/m10
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
Human Breast Epithelial Cell H184b5f5/M10, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp il17a hs00174383 m1
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
Gene Exp Il17a Hs00174383 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological v5 stk38 immunoprecipitates
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
V5 Stk38 Immunoprecipitates, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp il1b mm00434228 m1
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
Gene Exp Il1b Mm00434228 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chem Impex International kcl
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
Kcl, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher gene exp igf1 hs01555481 m1
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
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96
Tecan Systems positive pressure resolvex m10 96
Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and <t>OSR1,</t> and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; <t>OSR1,</t> odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.
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Image Search Results


Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and OSR1, and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; OSR1, odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.

Journal: Nature biomedical engineering

Article Title: Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip.

doi: 10.1038/s41551-017-0069

Figure Lengend Snippet: Figure 2 | Human iPS-cell-derived podocytes express markers characteristic of the mature phenotype. a, Representative fluorescence microscopy images of intermediate (int.) mesoderm, hiPS-cell-derived podocytes, and human immortalized podocytes stained for PAX2, WT1 and nephrin. b, Quantification of hiPS-cell-derived podocytes indicates upregulation of podocyte markers (nephrin, WT1 and podocin), with a corresponding decrease in the pluripotency marker OCT4. The decrease in progenitor cell markers PAX2 and OSR1, and the lack of EdU incorporation in hiPS-cell-derived podocytes indicate that the cells are post-mitotic and terminally differentiated, similar to mature podocytes. HPF, high-power field; EdU, 5-ethynyl-2′-deoxyuridine; OSR1, odd-skipped related transcription factor protein 1. c, Immunofluorescence microscopy images of hiPS-cell-derived podocytes and immortalized human podocytes stained for PKCλ/ι, a putative trafficker of nephrin to cell surface. d, Western blot analysis of total and phosphorylated (p-) PKCλ/ι proteins levels in hiPS cells, hiPS-cell-derived podocytes and human immortalized podocytes. e, Quantification of phosphorylated PKCλ/ι levels from western blots. Scale bars, 100 μm. Data are mean ± s.d.; n = 3 (b,e); *P < 0.05; **P < 0.001; ***P < 0.0001.

Article Snippet: The primary antibodies used included β 1 integrin (Abcam, ab24693), OCT4 (R&D Systems, AF1759), TRA-1-60 (Abcam, ab16288), goosecoid (R&D Systems, AF4086), brachyury (Abcam, ab20680), HAND1 (Abcam, ab196622), PAX2 (Invitrogen, 71-6000), OSR1 (Novus Biologicals, H00130497-M04), WT1 (Millipore, MAB4234), nephrin (Progen, GP-N2), podocin (Abcam, ab50339), VE-cadherin (Santa Cruz Biotech, sc-9989), PKCλ /ι (Santa Cruz Biotech, sc-727), collagen type IV (Abcam, ab6586) and FcRn (Santa Cruz Biotech, sc-271745).

Techniques: Derivative Assay, Fluorescence, Microscopy, Staining, Marker, Immunofluorescence, Western Blot